Ankyrons™

the future of binding reagents beyond antibodies

 

Ankyron Catalog

Custom Ankyrons

MHC-Peptide-Specific Ankyrons

Ankyrons Binding to Therapeutic Antibody Idiotypes

 

What are Ankyrons™?

Ankyrons™ are next-generation target binding reagents provided by ProImmune that overcome many of the current constraints of research antibodies.

Ankyrons disconnect from the animal-immune response paradigm of antibodies as binding reagents.

All Ankyrons are based on single domain ankyrin-repeat scaffold, selected in vitro by ribosome display for medium to high affinity from ProImmune’s synthetic Ankyron Teralibrary™ of around one trillion (~1012) clones. Therefore Ankyrons™ are by default recombinant sequence defined target binding molecules, unlike standard monoclonal antibodies. The exceptionally large starting clone number in our Teralibrary™ makes selecting Ankyrons more efficient than selecting antibody fragments from comparable antibody screening libraries in vitro.

Figure 1: Domain comparison between Ankyons and Antibodies. Ankyrons are highly stable recombinant single-domain binders. They can be directly labelled or detected via an epitope or biotin tag. Ankyrons can be engineered to combine them with further Ankyron or other domains much more easily than even recombinant Antibodies. Unlike Antibodies, Ankyrons do not have isotypes or any species origin.

Ankyron™ features

  • Always recombinant, monoclonal, sequence defined by default
  • Selected entirely in vitro from our Teralibrary™ for higher affinity
  • Unconstrained by immunological tolerance
  • Superior single domain properties compared to antibodies
  • Fast, inexpensive, customization with affinity tags and labels for every order
  • Rapidly growing catalog range to very wide range of targets and target areas
  • Custom recombinant monoclonal generation for any budget*
  • Further formatting to order as multi-specifics and multivalent molecules available
  • More cost-effective than research antibody technology in every respect
  • Generated without animal immunization
  • Distinguish closely related proteins with Ankyron guided selection
  • For research applications, including: flow cytometry, ELISA, western blotting, immunohistochemistry, immunoprecipitation, cell culture and more

*Contact us to discuss your options, including our reduced cost or even free of charge priority access program or Request a Quote (and we will respond within 1 working day).

You will be able to get Ankyrons for almost any target with and label, custom-configured every time with a plethora of formatting and customization options, including for bi-and multi-specific molecules, combined with a full range of labeling options, covering most types of available fluorochromes and other labels.

Any Ankyron is always available with any tag and label option we offer as standard.

Unlike catalog research antibodies, our catalog Ankyrons can be customized rapidly to your evolving requirements. Ankyrons help you move away from the primary/secondary antibody paradigm that still exists for most antibodies except those for the most common targets.

Our catalog range includes more than 20,000 clones for over 1,100 target proteins so far.

Formatting options for Ankyrons™ feature

Our catalog single-domain Ankyrons™ come with a 6His-tag and a V5 epitope tag by default. The customer has the choice of swapping the V5 epitope tag to the following alternative tags:

Alternative tags: Flag, C-Myc, HA, StII(WSHPQFEK), Biotin tag.

All Ankyrons™ are available with all of our labels as standard

In order to label Ankrons™ We have developed Alphalux™ labeling technology for consistent labeling of all Ankyrons with the following:

Standard covalent labels: Alphalux™ 405, 488, 532, 555, 647, 680, R-PE, APC, HRP

Note: Alphalux™ 405, 488, 532, 555, 647, 680 are spectrally equivalent to Alexafluor® colors.

Standard streptavidin (SA) labels via 1:1 conjugation of biotinylated Ankyron to:

SA-PE, SA-APC, SA-PE-Cy5, SA-PE-Cy7, SA-APC-Cy7, SA-HRP

Easy, fast, and affordable custom-labeling with every order:

Ankyrons can be conveniently labeled to order with any standard covalent fluorescent label or with any other standard streptavidin or avidin conjugate for minimal extra costs. It won’t take much longer either. If the label you want is not in the list above, please contact us for rapid turnaround no-obligation custom quote. Have it your way, every time!

With Ankyrons the primary vs secondary and catalog vs custom concepts either merge or become redundant.

Ankyrons blur the divide that has existed between traditional catalog and custom antibody products. Even if you don’t have the target protein you are interested in we may be able to make it, or a recombinant fragment thereof, for you either at no or low cost, if it is a publicly known target of general interest. We offer a privileged access program to free of charge custom made reagents, including raising Ankyrons to new targets in areas that are of sufficient interest to ProImmune. If you are interested in discussing this program, please contact us.

Find out more about custom Ankyrons.

 
Ankyron Guided Selection is like a superpower allowing you to drill into to the specificity you need

Ankyrons are selected directly in vitro from our Teralibrary™. In our discovery process we can easily screen Ankyron binders for selective reactivity with one type of target over closely related versions of the same target. Prominent examples include peptide-specific Ankyrons to peptide-MHC molecules and idiotype specific Ankyrons to antibodies, such as drug antibodies.

Figure: Guided selection discriminates which Ankyrons bind to specific variants of a molecule.

 
With Ankyrons (almost everything) comes within reach!

Research antibodies exist for many human targets and to a much lesser extent for targets from other species. Many gaps in coverage however still exist for many important research areas, due to the cost of raising antibodies, especially monoclonal and recombinant antibodies. Ankyrons are fundamentally suited for efficient and cost effective high throughput discovery, including to challenging targets such as peptide-MHC molecules. Contact us for the best options for custom discovery.

Humans exist among a universe of species. We need to find out more about them to find out more about ourselves. Affordable high-quality affordable new catalog and custom Ankyrons help you to explore more, faster, and with more confidence.

New Ankyron discovery is at the heart of the Ankyron story. Supporting discovery in key interest areas requires access to high quality antigen that is specifically configured for Ankyron discovery. To support this effort we have developed an internal high-throughput antigen provisioning platform which we call AnkyronX. AnkyronX can deliver hundreds of proteins for target discovery quickly and cost-effectively.

Ankyrons are automatically selected for high affinity

When we discover new target binding Ankyrons from our library we automatically only select for high affinity binders through our process. The typical affinity of most Ankyrons for their target is therefore at least in the nanomolar range and often we achieve picomolar affinity.

Figure: Affinities for Ankyrons can be determined for example by solution equilibrium titration assay.

Comparing Ankyrons™ to antibodies side by side

 Ankyrons™Antibodies
Size (MW)~15kD single domain~150kD, 12 domains (IgG)
SourceDirect selection from Teralibrary™ in vitroImmunization or selection from antibody library in vitro
Species/IsotypeNot originating from different species / no isotypesVarious species origins, various isotypes per species
StabilityHighly stable single domains
Can be highly stable for whole antibodies
EngineeringVery easy with single small domains
Possible, but more complex
Genetic definitionMonoclonal, recombinant by default
Varies, but getting from discovery to full formatted recombinant still expensive
CustomizationCan be ordered as custom conjugates every time; delivered in days not months
Custom conjugation possible, but typically significant extra cost; custom reagents often have long lead times
AffinityTypically high with direct selection from Teralibrary, bringing down the cost of high affinity reagents
Can be high if either generated as monoclonal in large animal or following selection and further optimization