ProM2® Class II MHC Monomers are pre-biotinylated and can be readily adapted either to detect and separate single-antigen specific T cells or implement specialist solid or solution phase immunoassays for measuring interactions of predefined MHC Class II-peptide complexes with ligands such as monoclonal antibodies, recombinant T cell receptors, or other binding scaffolds, e.g. for use in screening of novel antigen-specific CAR T cell antigen receptors.
ProM2® MHC Monomers are made by ProImmune’s experienced team who have been providing superior MHC reagents for more than 15 years now, including our world-leading Pro5® MHC Pentamers. Catalog ProM2™ Monomers are dispatched within 1-2 days for stock items, and 3 weeks for available items.
PBMC cultured with peptide Negative contol
Figure 1. PBMC from healthy donor stained with DR1/HA tetramers made from ProM2® Monomers of the same specificity.
Traditional functional assays for studying antigen-specific CD4+ T cells, such as proliferation or cytokine secretion, are not truly antigen-specific. The detection of CD4+ T cells has been difficult, due to the low frequency of these T cells (down to 1 in 100,000 white blood cells) and the low avidity of binding between the MHC-peptide binding complex and the T cell receptor. The optimized manufacturing process for ProM2® Class II MHC Monomers helps to address these issues by improving reproducibility and sensitivity of CD4+ T cell detection.
ProImmune provides ProM2® Class II MHC Monomers with responsive, expert technical support and customer service. Contrary to ordering class II tetramers from in-house or non-commercial facilities, customers do not need to apply and wait for reagents or supply any peptide. ProM2® Class II MHC Monomers are delivered quickly with stringent quality control and low batch-to-batch variability. As such ProM2® Class II MHC Monomers complement ProImmune’s existing Pro5® Class I Pentamer technology for detecting antigen-specific CD8+ T cells.
35ug, 100ug; please contact us for larger quantties
ProM2® Class II MHC Monomers are delivered in kit form with a choice of standard R-PE or APC labelled streptavidin to conjugate to. Alternatively customers can conjugate ProM2® Monomers to any other suitable avidin or streptavidin reagent.
A broad range of MHC Class II complexes specific for a wide range of infectious diseases such as Influenza, CMV, hepatitis B and C, HIV, EBV, as well as cancer-related epitopes are available as ProM2® Monomers. Catalog ProM2® Monomers are despatched in 1-2 days for Stock items, and 3 weeks for Available items.
Custom ProM2® Monomers for your allele/peptide of interest are available within approximately 6-7 weeks from ordering. Peptide sequences are subject to prior evaluation by ProImmune for binding. Once a peptide sequence is accepted for custom synthesis we will synthesize both the peptide and MHC monomer reagent.
HLA-DR1 (DRB1*01:01); DR2 (DRB1*15:01); DR3 (DRB1*03:01); DR4 (DRB1*04:01); DRB1*04:02 DRB1*04:05; DR5 (DRB1*11:01); DR7 (DRB1*07:01); DR9 (DRB1*09:01)
ProM2® Class II MHC Monomers can be used together with other Class II Tetramer reagents which customers may already have established in their labs.