Detecting and targeting specific MHC-peptide complexes is of material interest for understanding many disease conditions better and for developing novel therapeutic interventions. It has been very challenging to develop antibodies that bind selectively to an MHC-peptide combination over a more general MHC allotype.
Ankyrons provide a clear and powerful solution to this problem. Selected directly in vitro from our Teralibrary we can already offer a growing range of more than 16 of the most popular MHC-peptide combinations in cancer and infectious diseases in the listing below. Using guided selection we are able to select sequence specific Ankyrons quickly. ProImmune is also the world leader by publications from a commercial source in MHC-peptide multimer technology. So building MHC-peptide antigens for new Ankron discovery is already our core business.
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Data for MHC-peptide specific Ankyron staining
Figure. Staining of cell line constitutively expressing an infectious disease-specific MHC-peptide complex combination with Ankyrons selected to specifically bind the same (MHC-peptide specificity and study background are still subject to publication). Unstained data show background fluorescence. The cell line was stained with MHC-peptide specific Ankyrons as well as Ankyrons specific to an irrelevant peptide in the context of the same MHC allele. Staining was carried out in each case with and without additional stimulation with IFNγ. Stimulation with IFNγ strongly upregulates MHC-peptide presentation. In stimulated cells the staining of the cell line with the specific Ankyron separates clearly from that of the non specific Ankyron staining. Data provided by Prof. Antonio Bertoletti’s group at Duke NUS, Singapore.