Pro5^® MHC Class I Pentamers for Simian Alleles

MHC class I-restricted CD8+ T cell responses are important for controlling virus replication in both human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection. Since macaque and human MHC class I molecules have been found to bind peptides derived from similar regions of the Gag and Env proteins of HIV and SIV, detailed studies of the rhesus macaque CD8+ T cell response to SIV infection are of considerable benefit to our understanding of the process of HIV infection and successful vaccine development.

Simian Pentamer staining protocol (PDF)

Advantages of Pro5® Pentamers

Applications of Pro5® Pentamers

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In order to enhance SIV research, ProImmune offers Pro5® MHC Class I Pentamers for simian alleles Mamu-A*01 and Mamu-A*02 for use in flow cytometry, to detect and separate antigen-specific CD8+ T cells in the rhesus macaque model. Pro5® Pentamers deliver consistent and reproducible results and are suitable for detailed epitope characterization, as well as analysis on a single cell level when used in combination with intracellular cytokine staining.

Experimental data from Tulane National Primate Research Center, Covington, LA, USA.

ProImmune’s Mamu-A*01 and Mamu-A*02 Pro5® Pentamers were tested by Dr. Marcelo Kuroda at the Tulane National Primate Research Center (USA).

Mamu-A*01-restricted Pro5® Pentamer containing peptide derived from SIV Gag 181-189 (CTPYDINQM), and Mamu-A*02-restricted Pro5® Pentamers containing peptides derived from SIV Nef 159-167 (YTSGPGIRY) and SIV Gag 71-79 (GSENLKSLY), were tested on whole blood from SIVmac251-infected Mamu-A*01+/Mamu-A*02+ rhesus macaques. Cells were incubated with 1 test of R-PE- or APC-labeled Pentamer for 10 minutes at room temperature (22ºC). Following washing, cells were incubated with 1 test anti CD8-PerCP (clone SK1) for 20 minutes at 4ºC. Following a further 2 washes, cells were fixed for analysis by flow cytometry.

Simian Allele Products

The table shows the Pro5® Pentamer specificities that are available as catalog items, with a dispatch time of 5-10 working days. Pro5® Pentamers are available with R-PE, APC or biotin label.  Alternatively, an unlabeled Pentamer can be ordered along with a Pro5® Fluorotag (R-PE or APC) or Pro5® Biotag (biotin label). Unlabeled Pentamers are perfect for long term studies as they are guaranteed for 12 months when stored at -80ºC. The corresponding matched peptide can also be ordered in 1 mg or 2 mg quantity.

Alternatively, a Custom Pro5® Pentamer with Mamu-A*01 or Mamu-A*02 can be made to your specification in 4-6 weeks.