Pro5® MHC Class I Pentamers
the most consistent, most published commercial technology for detecting antigen-specific CD8+ T cells
Figure 1. Pro5® Pentamer. The beautiful rational design of Pro5® MHC Pentamers presents five MHC peptide complexes in a plane at one end of their pentameric coiled-coil core and five detection tags in a second plane at the opposite end of the core.
Pro5® Pentamers feature
Example Published Pro5® Pentamer Staining Data:
Figure 2. CD8+ T cells specific for the tumor antigen NY-ESO-1 are predominantly CD103+. Flow cytometry analysis of T cells derived from the malignant ascites of an HLA-A2+ EOC patient (IROC013) who demonstrated reactivity to an HLA-A2-restricted epitope of NY-ESO-1. The analysis shows the frequency of CD8+ lymphocytes staining positive with an A*02:01/SLLMWITQV (NY-ESO-1157–165) Pro5® Pentamer. Pentamer-positive CD8+ T cells were further characterized for CD103 expression (right panel). All events were first gated on total lymphocyte populations by forward and side scatter.
Pro5® Pentamers: consitency by design for the most demanding applications
When you are trying to detect a rare population of antigen specific T cells you will understand the importance of having a research tool that is sensitive, accurate and gives reproducible results. Pro5® MHC Class I Pentamers are designed for these objectives. They bind directly to T cell receptors of a particular specificity, determined by the Major Histocompatibility Complex (MHC) allele and peptide combination. Pro5® Pentamers can be used to detect and separate antigen-specific CD8+ T cell populations as rare as 0.02% of lymphocytes. They are also suitable for detailed epitope characterization and further immune monitoring.
Unlike MHC multimers from other sources, which can be of variable quality and stability, Pro5® Pentamers benefit from a superior design and are made to a professional standard. Each Pentamer is purified four times before being subjected to several stringent quality control steps. Using the Pentamer technology enables you to achieve accurate and reproducible results.