Pro5® MHC Class I Pentamers
The most consistent technology for detecting antigen-specific T cells
If you are trying to detect a rare population of antigen specific T cells you will understand the importance of having a research tool that is sensitive, accurate and gives reproducible results.
Pro5® MHC Class I Pentamers provide the answer. They bind directly to T cell receptors of a particular specificity, determined by the Major Histocompatibility Complex (MHC) allele and peptide combination. Pro5 Pentamers can be used to detect and separate antigen-specific CD8+ T cell populations as rare as 0.02% of lymphocytes. They are also suitable for detailed epitope characterization and further immune monitoring.
Unlike tetramers from academic and non-profit sources, which may be of variable quality and stability, Pro5 Pentamers benefit from a superior design and are made to a professional standard including many stringent quality control steps. Using the Pentamer technology enables you to achieve accurate and reproducible results.
Pro5® Pentamers comprise five MHC-peptide complexes assembled through a coiled-coil domain. Due to their planar configuration, all five MHC-peptide complexes in the Pentamer are available for binding to complementary T cell receptors (TCRs). Each Pro5® Pentamer also comprises up to five fluorescent or biotin tags for bright and efficient labeling. In contrast, the MHC tetramer technology relies on MHC-peptide complexes held in a tetrahedral complex where due to spatial organization no more than three MHC-peptide complexes are available for TCR-binding.
A broad range of MHC Class I complexes specific for a wide range of infectious diseases such as Influenza, CMV, hepatitis B and C, HIV, EBV, as well as cancer-related epitopes are available as Pro5® Pentamers. Simian alleles, Mamu-A*01 and Mamu-A*02 are available for SIV research. Click here to view our full range of almost 300 Pentamers. Additionally, ProImmune has many years experience making Custom MHC Pentamers, specific to customers' requirements and with a high rate of success.
ProImmune has extensive experience in testing Pro5® Pentamer products in-house in a variety of applications. This enables us to support our customers fully with their relevant application requirements, helping them to make best use of our technology.
Figure: MHC multimer staining vs. CD8 FITC of peripheral blood (PBMC) samples. 1 x 105 PBMCs with specificity for each antigen shown were stained with either Pro5® Pentamer or MHC tetramer. The figure shows live cells and demonstrates the sensitivity and increase in mean fluorescence intensity when cells are stained with Pentamer vs. tetramer.