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Ankyron VGFR2_MOUSE Clone BC40280
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BC40280 binding to 0.5 picomoles of immobilised VGFR2_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Whole mount immunostaining of formaldehyde-fixed (1 % v/v in PBS) mouse ear tissue using 5 μg V5-tagged Ankyron and 8 μL anti-V5 APC Fluorotag for Ankyron.
Whole mount immunostaining of formaldehyde-fixed (1 % v/v in PBS) mouse ear tissue using 5 μg V5-tagged Ankyron and 8 μL anti-V5 APC Fluorotag for Ankyron.