Ankyron B2CL1_HUMAN Clone BC40274

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Price £245.00
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Clone Information

Clone ?

BC40274
Gene

BCL2L1 BCL2L BCLX
UniProtID

Q07817
Organism

Homo sapiens (Human)
Entry Name

B2CL1_HUMAN
SNR ?

626800
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

MCF-7 were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BC40274 binding to 0.5 picomoles of immobilised B2CL1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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