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Ankyron UBE2T_HUMAN Clone DQ40278
Clone Information
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Clone ?
|
DQ40278 |
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Gene
|
UBE2T HSPC150 PIG50 |
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UniProtID
|
Q9NPD8 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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UBE2T_HUMAN |
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SNR ?
|
1319900 |
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Product Type
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Monomer |
|
Ankyron Applications
|
IF, IHC, ELISA, Kd |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded colorectal cancer using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DQ40278 binding to 0.5 picomoles of immobilised UBE2T_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DQ40278 binding to UBE2T_HUMAN, of value Kd = 17.3 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.