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Ankyron NFKB1_MOUSE Clone DQ40248
Clone Information
|
Clone ?
|
DQ40248 |
|
Gene
|
NFKB1 |
|
UniProtID
|
P25799 |
|
Organism
|
Mus musculus (Mouse) |
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Entry Name
|
NFKB1_MOUSE |
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SNR ?
|
8163 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DQ40248 binding to 0.5 picomoles of immobilised NFKB1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DQ40248 binding to NFKB1_MOUSE, of value Kd = 1640.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.