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Ankyron SFTPA_MOUSE Clone CA30166
Clone Information
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Clone ?
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CA30166 |
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Gene
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Sftpa1 Sftp-1 Sftp1 Sftpa |
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UniProtID
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P35242 |
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Organism
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Mus musculus (Mouse) |
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Entry Name
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SFTPA_MOUSE |
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SNR ?
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28 |
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Product Type
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Monomer |
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Ankyron Applications
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F, IF, ELISA, Kd |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
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Kd <100nM |
Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of mouse J774A.1 cells following permeabilisation with Cytofix/Cytoperm and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CA30166 binding to 0.5 picomoles of immobilised SFTPA_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CA30166 binding to SFTPA_MOUSE, of value Kd = 21.1 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.