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Ankyron PD1L1_MOUSE Clone CI30496
Clone Information
|
Clone ?
|
CI30496 |
|
Gene
|
CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 |
|
UniProtID
|
Q9EP73 |
|
Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
PD1L1_MOUSE |
|
SNR ?
|
6508 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
Flow cytometric analysis of mouse splenocytes following fixation in formaldehyde and staining with pre-conjugated Ankyron (0.4 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of mouse J774A.1 following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CI30496 binding to 0.5 picomoles of immobilised PD1L1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CI30496 binding to PD1L1_MOUSE, of value Kd = 50.2 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.