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Ankyron PD1L1_MOUSE Clone CI30483
Clone Information
|
Clone ?
|
CI30483 |
|
Gene
|
CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 |
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UniProtID
|
Q9EP73 |
|
Organism
|
Mus musculus (Mouse) |
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Entry Name
|
PD1L1_MOUSE |
|
SNR ?
|
5910 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, ELISA, Kd, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
Flow cytometric analysis of mouse mouse splenocytes following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of mouse mouse splenocytes following fixation in formaldehye and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1 µg) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CI30483 binding to 0.5 picomoles of immobilised PD1L1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CI30483 binding to PD1L1_MOUSE, of value Kd = 115.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.