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Ankyron P53_MOUSE Clone BT40422
Clone Information
|
Clone ?
|
BT40422 |
|
Gene
|
Tp53 P53 Trp53 |
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UniProtID
|
P02340 |
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Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
P53_MOUSE |
|
SNR ?
|
979 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were permeabilised with 0.5% Triton X-100, fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin (green). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BT40422 binding to 0.5 picomoles of immobilised P53_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BT40422 binding to P53_MOUSE, of value Kd = 1110.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.