Ankyron P53_MOUSE Clone BT40420

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Price £245.00
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Clone Information

Clone ?

BT40420
Gene

Tp53 P53 Trp53
UniProtID

P02340
Organism

Mus musculus (Mouse)
Entry Name

P53_MOUSE
SNR ?

1392
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His
Ankyron Kd

Kd Available

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells were permeabilised with 0.5% Triton X-100, fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin (green). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BT40420 binding to 0.5 picomoles of immobilised P53_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BT40420 binding to P53_MOUSE, of value Kd = 143.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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