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Ankyron KIT_MOUSE Clone AK40219
Clone Information
|
Clone ?
|
AK40219 |
|
Gene
|
Kit Sl |
|
UniProtID
|
P05532 |
|
Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
KIT_MOUSE |
|
SNR ?
|
1051 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were permeabilised with 0.5% Triton X-100, fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin (green). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AK40219 binding to 0.5 picomoles of immobilised KIT_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.