Your basket is currently empty!
Ankyron KIT_MOUSE Clone AK40217
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were permeabilised with 0.5% Triton X-100, fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin (green). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AK40217 binding to 0.5 picomoles of immobilised KIT_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.