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Ankyron KI67_HUMAN Clone DS40118
Clone Information
|
Clone ?
|
DS40118 |
|
Gene
|
MKI67 |
|
UniProtID
|
P46013 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
KI67_HUMAN |
|
SNR ?
|
4462 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Flow cytometric analysis of HCT116 (blue), HeLa (green) and HUVEC (red) following permeabilisation using Foxp3 fix-perm buffer, then incubation with pre-conjugated Ankyron (0.4 μg) and anti-V5 APC. Negative control, anti-V5 APC only (grey). HCT116 proliferate more rapidly than HeLa cells whereas HUVEC have a longer doubling time than either of the other cell lines, and thus lower levels of Ki67.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DS40118 binding to 0.5 picomoles of immobilised KI67_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.