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Ankyron KI67_HUMAN Clone DS40116
Ankyrons® Testing Data
HeLa were permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin (green). Inset: negative control of anti-V5 APC antibody alone.
J774A.1 mouse macrophages were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100, then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DS40116 binding to 0.5 picomoles of immobilised KI67_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Flow cytometric analysis of HeLa cells following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) and lysates from HeLa cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).