J774A.1 mouse macrophage cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (magenta) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (cyan).
Flow cytometric analysis of J774A.1 mouse macrophage cells following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AY40443 binding to 0.5 picomoles of immobilised JAM1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
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