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Ankyron IL34_HUMAN Clone AW40241
Clone Information
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Clone ?
|
AW40241 |
|
Gene
|
IL34 C16orf77 |
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UniProtID
|
Q6ZMJ4 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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IL34_HUMAN |
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SNR ?
|
801 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
HepG2 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Immunohistochemical analysis of paraffin-embedded human spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica EDTA buffer (pH9), 40 min.
Flow cytometric analysis of HepG2 cells following permeabilisation and staining with pre-conjugated Ankyron (0.4 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40241 binding to 0.5 picomoles of immobilised IL34_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.