Ankyron IL33_HUMAN Clone BG40315

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Price £245.00
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Clone Information

Clone ?

BG40315
Gene

IL33 C9orf26 IL1F11 NFHEV
UniProtID

O95760
Organism

Homo sapiens (Human)
Entry Name

IL33_HUMAN
SNR ?

8004
Product Type

Monomer
Ankyron Applications
F, IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His

Ankyrons® Testing Data

HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human HepG2 cells following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BG40315 binding to 0.5 picomoles of immobilised IL33_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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