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Ankyron IGF2_HUMAN Clone BC40372
Clone Information
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Clone ?
|
BC40372 |
|
Gene
|
IGF2 IGF-2 PP1446 |
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UniProtID
|
P01344 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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IGF2_HUMAN |
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SNR ?
|
4096 |
|
Product Type
|
Monomer |
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Ankyron Applications
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IF, ELISA |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
HCT116 were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BC40372 binding to 0.5 picomoles of immobilised IGF2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.