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Ankyron HIF1A_HUMAN Clone AW40111
Ankyrons® Testing Data
HUVEC were incubated with Cobalt (II) chloride hexahydrate overnight (to stabilise hypoxia inducible factors), then fixed in formaldehyde (4%) and permeabilised with 0.5% Triton X-100. Cells were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40111 binding to 0.5 picomoles of immobilised HIF1A_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.