Flow cytometric analysis of human dendritic cells following overnight stimulation with LPS, then staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AA40115 binding to 0.5 picomoles of immobilised CD80_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
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