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Ankyron CD80_HUMAN Clone AA40122
Ankyrons® Testing Data
Flow cytometric analysis of human dendritic cells either resting (orange) or following stimulation (red) overnight with LPS, prior to staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative controls, anti-V5 APC only on unstimulated (light grey) and stimulated (dark grey) cells.
Flow cytometric analysis of human dendritic cells, either resting (orange) or following stimulation (red) overnight with LPS, prior to fixation in formaldehyde (1 %) and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative controls, anti-V5 APC only on unstimulated (light grey) and stimulated (dark grey) cells.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AA40122 binding to 0.5 picomoles of immobilised CD80_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.