Ankyron CD14_MOUSE Clone AW40861

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Clone Information

Clone ?

AW40861
Gene

CD14
UniProtID

P10810
Organism

Mus musculus (Mouse)
Entry Name

CD14_MOUSE
SNR ?

212
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells (CD14-positive) and HUVEC (CD14-negative) were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 cells following incubation with preconjugated Ankyron (0.43 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only.
Flow cytometric analysis of mouse RAW264.7 cells following incubation with preconjugated Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only. Data courtesy of Victoria Richardson and Subhankar Mukhopadhyay, Kings College London.
Flow cytometric analysis of mouse splenocytes following incubation with antibodies against CD45, Ly6G, CD3 and preconjugated V5-tagged Ankyron with anti-V5 APC, titrated at 1 - 6 µg. CD14 was detected by Ankyron in CD3-negative splenocytes and absent from Ly6G-positive neutrophils, as expected. Data courtesy of Oscar Haigh, CEA Paris-Saclay.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40861 binding to 0.5 picomoles of immobilised CD14_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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