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Ankyron CD14_MOUSE Clone AW40859
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells (CD14-positive) and HUVEC (CD14-negative) were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 cells following incubation with preconjugated Ankyron (0.43 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only.
Flow cytometric analysis of mouse RAW264.7 cells following incubation with preconjugated Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only. Data courtesy of Victoria Richardson and Subhankar Mukhopadhyay, Kings College London.
Flow cytometric analysis of mouse splenocytes following incubation with antibodies against CD45, Ly6G, CD3 and preconjugated V5-tagged Ankyron with anti-V5 APC, titrated at 1 - 6 µg. CD14 was detected by Ankyron in CD3-negative splenocytes and absent from Ly6G-positive neutrophils, as expected. Data courtesy of Oscar Haigh, CEA Paris-Saclay.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40859 binding to 0.5 picomoles of immobilised CD14_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.