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Ankyron CCL2_HUMAN Clone AB30107
Ankyrons® Testing Data
HUVEC were stimulated with TNF-α for 20 hours, then fixed in formaldehyde (4%), permeabilised with 0.1% Triton X-100, then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB30107 binding to 0.5 picomoles of immobilised CCL2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.