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Ankyron CADH2_HUMAN Clone AE40401
Ankyrons® Testing Data
HCT116 cells were fixed in formaldehyde (4%), then stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of HCT116 cells, following incubation with 2.5 μg Ankyron and detecting with anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Colorimetric western blot analysis of purified target protein (100 - 10 ng) using 1 µg/ml V5-tagged Ankyron premixed with anti-V5-HRP antibody.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE40401 binding to 0.5 picomoles of immobilised CADH2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.