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Ankyron CADH1_HUMAN Clone AE40381
Clone Information
|
Clone ?
|
AE40381 |
|
Gene
|
CDH1 CDHE UVO |
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UniProtID
|
P12830 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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CADH1_HUMAN |
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SNR ?
|
16510 |
|
Product Type
|
Monomer |
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Ankyron Applications
|
IF, IHC, ELISA |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HCT116 cells were fixed in formaldehyde (4%), then stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
HCT116 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
IHC of longitudinally sectioned human colon, staining using the V5-tagged Ankyron clone ANK00519_4 and anti-V5 HRP with DAB (brown) and counterstaining nuclei with hematoxylin (purple). Negative control: anti-V5 HRP with DAB in absence of Ankyron.
Immunohistochemical analysis of paraffin-embedded human normal skin using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Citrate buffer (pH6).
Immunohistochemical analysis of paraffin-embedded human normal skin using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE40381 binding to 0.5 picomoles of immobilised CADH1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.