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Ankyron X3CL1_HUMAN Clone AJ30433
Ankyrons® Testing Data
HUVEC were cultured for 20 h in either the absence or presence of TNFα, then fixed in formaldehyde (4%) and stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in either Staining buffer (RPMI 1640 + 10 % human serum) for non-permeabilised samples or in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy.
HUVEC were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AJ30433 binding to 0.5 picomoles of immobilised X3CL1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.