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Ankyron VTNC_HUMAN Clone AN40209
Clone Information
|
Clone ?
|
AN40209 |
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Gene
|
VTN |
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UniProtID
|
P04004 |
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Organism
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Homo sapiens (Human) |
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Entry Name
|
VTNC_HUMAN |
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SNR ?
|
3647 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA, Kd, WB |
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Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Colorimetric western blot analysis of purified target protein (1 µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AN40209 binding to 0.5 picomoles of immobilised VTNC_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AN40209 binding to VTNC_HUMAN, of value Kd = 230.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.