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Ankyron VISTA_MOUSE Clone DT40333
Clone Information
|
Clone ?
|
DT40333 |
|
Gene
|
Vsir Dies1 PD-1H VISTA |
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UniProtID
|
Q9D659 |
|
Organism
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Mus musculus (Mouse) |
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Entry Name
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VISTA_MOUSE |
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SNR ?
|
70300 |
|
Product Type
|
Monomer |
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Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
J774A.1 mouse macrophage were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DT40333 binding to 0.5 picomoles of immobilised VISTA_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.