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Ankyron VGFR3_MOUSE Clone BD40135
Clone Information
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Clone ?
|
BD40135 |
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Gene
|
Flt4 Flt-4 Vegfr3 |
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UniProtID
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P35917 |
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Organism
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Mus musculus (Mouse) |
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Entry Name
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VGFR3_MOUSE |
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SNR ?
|
1904 |
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Product Type
|
Monomer |
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Ankyron Applications
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F, IF, ELISA |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Mouse macrophage J774A.1 cells (VGFR3-positive) and HUVEC (VGFR3-low, human cells) were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Whole mount immunostaining of formaldehyde fixed (1 % v/v in PBS) mouse ear tissue using 5 μg V5-tagged Ankyron and 8 μL anti-V5 APC Fluorotag for Ankyron.
Flow cytometric analysis of mouse J774A.1 cells following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of mouse J774A.1 cells following staining with pre-conjugated Ankyron (0.22 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BD40135 binding to 0.5 picomoles of immobilised VGFR3_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.