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Ankyron VCAM1_HUMAN Clone AB40529
Ankyrons® Testing Data
HUVECs were cultured for 24 h in either endothelial growth cell medium alone (control, untreated) or in the presence of TNFα, then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Images captured by epifluorescence microscopy.
HUVEC were cultured for 24 h in the presence of TNFα, then fixed in formaldehyde prior to staining for flow cytometry with 2.5 μg Ankyron and anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40529 binding to 0.5 picomoles of immobilised VCAM1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.