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Ankyron VCAM1_HUMAN Clone AB40529
Clone Information
|
Clone ?
|
AB40529 |
|
Gene
|
VCAM1 |
|
UniProtID
|
P19320 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
VCAM1_HUMAN |
|
SNR ?
|
129906 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HUVECs were cultured for 24 h in either endothelial growth cell medium alone (control, untreated) or in the presence of TNFα, then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Images captured by epifluorescence microscopy.
HUVEC were cultured for 24 h in the presence of TNFα, then fixed in formaldehyde prior to staining for flow cytometry with 2.5 μg Ankyron and anti-V5-APC, (red). Negative control: anti-V5-APC alone, with no Ankyron present (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40529 binding to 0.5 picomoles of immobilised VCAM1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.