Ankyron VCAM1_HUMAN Clone AB40522

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Price £245.00
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Clone Information

Clone ?

AB40522
Gene

VCAM1
UniProtID

P19320
Organism

Homo sapiens (Human)
Entry Name

VCAM1_HUMAN
SNR ?

430412
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

HUVECs were cultured for 24 h in the presence of TNFα, then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Images captured by epifluorescence microscopy.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40522 binding to 0.5 picomoles of immobilised VCAM1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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