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Ankyron UBAC1_HUMAN Clone BM30104
Ankyrons® Testing Data
HUVEC were stained using 2.5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BM30104 binding to 0.5 picomoles of immobilised UBAC1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BM30104 binding to UBAC1_HUMAN, of value Kd = 369.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.