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Ankyron TSP1_MOUSE Clone CJ30457
Clone Information
|
Clone ?
|
CJ30457 |
|
Gene
|
Thbs1 Tsp1 |
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UniProtID
|
P35441 |
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Organism
|
Mus musculus (Mouse) |
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Entry Name
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TSP1_MOUSE |
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SNR ?
|
19165 |
|
Product Type
|
Monomer |
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Ankyron Applications
|
IF, ELISA |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
J774A.1 were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CJ30457 binding to 0.5 picomoles of immobilised TSP1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.