Ankyron TNR4_MOUSE Clone AO40619

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Clone Information

Clone ?

AO40619
Gene

Tnfrsf4 Ox40 Txgp1
UniProtID

P47741
Organism

Mus musculus (Mouse)
Entry Name

TNR4_MOUSE
SNR ?

2912
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 cells, either resting (blue) or following stimulation (red) overnight with LPS, prior to staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative controls, anti-V5 APC only on unstimulated (light grey) and stimulated (dark grey) cells.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40619 binding to 0.5 picomoles of immobilised TNR4_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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