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Ankyron TNR18_MOUSE Clone AS40119
Clone Information
|
Clone ?
|
AS40119 |
|
Gene
|
Tnfrsf18 Gitr |
|
UniProtID
|
O35714 |
|
Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
TNR18_MOUSE |
|
SNR ?
|
437 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 cells following staining with pre-conjugated Ankyron (0.2 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AS40119 binding to 0.5 picomoles of immobilised TNR18_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.