Ankyron TNF14_HUMAN Clone AC40577

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Price £245.00
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Clone Information

Clone ?

AC40577
Gene

TNFSF14 HVEML LIGHT UNQ391/PRO726
UniProtID

O43557
Organism

Homo sapiens (Human)
Entry Name

TNF14_HUMAN
SNR ?

12965
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His, Biotin (V5, His), FLAG (V5, His), Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His)

Ankyrons® Testing Data

Monocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of permeabilised monocytes following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AC40577 binding to 0.5 picomoles of immobilised TNF14_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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