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Ankyron TNF13_HUMAN Clone BV40709
Clone Information
|
Clone ?
|
BV40709 |
|
Gene
|
TNFSF13 APRIL TALL2 ZTNF2 UNQ383/PRO715 |
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UniProtID
|
O75888 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
TNF13_HUMAN |
|
SNR ?
|
2890 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, IHC, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Monocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human colon using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1:500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Citrate buffer (pH6).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BV40709 binding to 0.5 picomoles of immobilised TNF13_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.