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Ankyron THRB_PIG Clone FT40148
Clone Information
|
Clone ?
|
FT40148 |
|
Gene
|
F2 |
|
UniProtID
|
Q19AZ8 |
|
Organism
|
Sus scrofa (Pig) |
|
Entry Name
|
THRB_PIG |
|
SNR ?
|
498 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
L45 porcine mononuclear cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of L45 porcine mononuclear cells following permeabilisation with Cytofix/Cytoperm and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® FT40148 binding to 0.5 picomoles of immobilised THRB_PIG, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.