Ankyron THIOM_HUMAN Clone AT30122

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Clone Information

Clone ?

AT30122
Gene

TXN2 TRX2
UniProtID

Q99757
Organism

Homo sapiens (Human)
Entry Name

THIOM_HUMAN
SNR ?

5194
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

HUVECs were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Colorimetric western blot of recombinant protein, using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP antibody. We cannot guarantee that this Ankyron will detect protein within cell lysate.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AT30122 binding to 0.5 picomoles of immobilised THIOM_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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