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Ankyron TGFB1_HUMAN Clone AE50275
Clone Information
|
Clone ?
|
AE50275 |
|
Gene
|
TGFB1 TGFB |
|
UniProtID
|
P01137 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
TGFB1_HUMAN |
|
SNR ?
|
166 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HeLa cells were fixed in formaldehyde (4%), then stained using 10 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilization Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE50275 binding to 0.5 picomoles of immobilised TGFB1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.