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Ankyron TF65_HUMAN Clone AW40912
Ankyrons® Testing Data
HUVEC were cultured overnight in the presence of TNFα. Cells were permeabilised with 0.1% Triton X-100, then stained using 2.5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and F-actin with Phalloidin. Image captured by epifluorescence microscopy. Insets: negative control of anti-V5 APC antibody alone, with DAPI and F-actin counterstaining.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40912 binding to 0.5 picomoles of immobilised TF65_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.