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Ankyron TCF7_HUMAN Clone CD30332
Clone Information
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Clone ?
|
CD30332 |
|
Gene
|
TCF7 TCF1 |
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UniProtID
|
P36402 |
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Organism
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Homo sapiens (Human) |
|
Entry Name
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TCF7_HUMAN |
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SNR ?
|
269 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA, Kd |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CD30332 binding to 0.5 picomoles of immobilised TCF7_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CD30332 binding to TCF7_HUMAN, of value Kd = 694.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.