Ankyron TCAM1_HUMAN Clone EM30642

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Price US$307.00
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Clone Information

Clone ?

EM30642

Gene

TICAM1 PRVTIRB TRIF

UniProtID

Q8IUC6

Organism

Homo sapiens (Human)

Entry Name

TCAM1_HUMAN

SNR ?

272

Product Type

Monomer

Ankyron Applications
IF, ELISA, Kd
Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His
Ankyron Kd

Kd Available

HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® EM30642 binding to 0.5 picomoles of immobilised TCAM1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® EM30642 binding to TCAM1_HUMAN, of value Kd = 407.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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