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Ankyron SUMO1_HUMAN Clone DC40703
Clone Information
|
Clone ?
|
DC40703 |
|
Gene
|
SUMO1 SMT3C SMT3H3 UBL1 OK/SW-cl.43 |
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UniProtID
|
P63165 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
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SUMO1_HUMAN |
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SNR ?
|
3857 |
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Product Type
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Monomer |
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Ankyron Applications
|
IF, IHC, ELISA |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Immunohistochemical analysis of paraffin-embedded human tonsil using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica EDTA buffer (pH9), 40 min.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DC40703 binding to 0.5 picomoles of immobilised SUMO1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.