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Ankyron STAT1_MOUSE Clone CK30296
Clone Information
|
Clone ?
|
CK30296 |
|
Gene
|
STAT1 |
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UniProtID
|
P42225 |
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Organism
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Mus musculus (Mouse) |
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Entry Name
|
STAT1_MOUSE |
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SNR ?
|
1707 |
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Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, Kd |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
J774A.1 were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CK30296 binding to 0.5 picomoles of immobilised STAT1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CK30296 binding to STAT1_MOUSE, of value Kd = 12.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.